Host tree resistance against the polyphagous wood-boring beetle Anoplophora glabripennis

Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae: Lamiini) is an invasive wood‐boring beetle with an unusually broad host range and a proven ability to increase its host range as it colonizes new areas and encounters new tree species. The beetle is native to eastern Asia and has become an invasive pest in North America and Europe, stimulating interest in delineating host and non‐host tree species more clearly. When offered a choice among four species of living trees in a greenhouse, adult A. glabripennis fed more on golden‐rain tree (Koelreuteria paniculata Laxmann) and river birch (Betula nigra L.) than on London planetree (Platanus × acerifolia (Aiton) Willdenow) or callery pear (Pyrus calleryana Decaisne). Oviposition rate was highest in golden‐rain tree, but larval mortality was also high and larval growth was slowest in this tree species. Oviposition rate was lowest in callery pear, and larvae failed to survive in this tree species, whether they eclosed from eggs laid in the trees or were manually inserted into the trees. Adult beetles feeding on callery pear had a reduced longevity and females feeding only on callery pear failed to develop any eggs. The resistance of golden‐rain tree against the larvae appears to operate primarily through the physical mechanism of abundant sap flow. The resistance of callery pear against both larvae and adults appears to operate through the chemical composition of the tree, which may include compounds that are toxic or which otherwise interfere with normal growth and development of the beetle. Unlike river birch or London planetree, both golden‐rain tree and callery pear are present in the native range of A. glabripennis and may therefore have developed resistance to the beetle by virtue of exposure to attack during their evolutionary history.     

Turfgrass (Cynodon dactylon L.) sod production on sandy soils: II. Effects of irrigation and fertiliser regimes on N leaching

The effects of irrigation and fertiliser regimes on N leaching from the production of couch grass (Cynodon dactylon L.) sod, on a free-draining sandy soil, were evaluated in a 22-month field study. The experimental design used a randomised-block, split-plot design with three replicates. Main plots consisted of two irrigation treatments: 70 and 140% daily replacement of pan evaporation; four subplot fertiliser types (water-soluble (predominately NH₄NO₃), control-release, pelletised poultry manure and pelletised biosolids); and three N application rates (100, 200 and 300 kg N ha⁻¹ per crop). Nitrogen leaching was assessed by measuring the leachate volumes and concentrations of N species leached from soil lysimeters (250 mm in diameter by 950 mm in length) installed in 10 m² turfgrass plots. Nitrogen leaching ranged from 33 to 167 kg N ha⁻¹ over 22 months, depending upon the irrigation and fertiliser treatment. Irrigation treatment affected N leaching more than fertiliser treatment, and increasing the irrigation from 70 to 140% replacement of daily pan evaporation increased N leaching for all fertiliser types, and by up to four times. Forty six to 76% of losses occurred from the high irrigation treatments during the first 16 weeks after the turfgrass was planted as rhizomes. By contrast, N leaching did not appear to increase following harvest of sod. At the high irrigation treatment, N leaching was greater for the pelletised biosolids than the control-release; while at the low irrigation treatment, N leaching did not vary between fertiliser types. A significant proportion of the N leached was in the organic form. Therefore, we recommend total N and mineral N be measured when assessing N leaching from turfgrass. Nitrogen leaching from turfgrass production is low from all fertiliser types when the irrigation matches turfgrass water use and N is applied at a rate and frequency that approximates turfgrass requirements. Section Editor: P. J. Randall     

Aldrin epoxidation by plant root extracts

Aldrin epoxidase activity of the cell-free pea and bean root preparations was located in the particulate fraction. High speed centrifugation at 250 000 g for 2 hr resulted in a pellet with almost all the activity of the crude cell-free preparations. While the epoxidase was stimulated by NADPH generating system in these cell-free root preparations, that in high speed centrifugation pellets was not. Aldrin epoxidase activity of the dwarf bean root homogenates was increased by addition of p-aminobenzoic acid (10^?4 M). This increase in activity is above that already manifested by Polyclar AT. No activity was detected in the dormant or germinating Alaska peas or dwarf beans until the 5th and 6th day, respectively.     

Fusinite of fossil coals as an information source about the anatomy of ancient plants

“Clarification” and ion and high-frequency etching of fusinite are described. These methods allow microscopic examination of fusinitized plant tissues in fine anatomical detail, revealing the outlines of cells, the structure of cell walls, the outlines of bordered pits, and, occasionally, even cellular microstructures. It is supposed that the effects of “clarification” and etching are caused by those optical differences between the composition of organic biopolymers that constituted the anatomical structures of plants that have been preserved in the fossil state.     

What is the optimal number of leaves when measuring leaf area of tree species in a forest community?

植物形态性状叶面积简单易测, 能够反映植物对环境的适应与响应, 指示生态系统的功能与过程。在野外测定叶面积时, 叶片取样数量往往采用约定俗成的10-20片, 但到底采集多少叶片才是最优和最具代表性, 却少有探究。该研究以浙江金华山常绿落叶阔叶混交林的优势树种木荷(Schima superba)与枫香树(Liquidambar formosana)为研究对象, 通过对5个胸径等级植株和每个植株6个方位开展大批量叶片取样(>2 500个), 分析两个树种的叶面积变异特征, 探讨叶片取样数量为多少才能最代表该森林类型的叶片大小性状规律。结果表明, 常绿乔木木荷平均叶面积与变幅均小于落叶乔木枫香树。木荷叶面积与胸径无显著相关性, 而枫香树叶面积与胸径有较显著相关性, 但两个树种均在中胸径等级(15-20 cm)差异不显著; 两个树种的叶面积与采样方位无显著相关性, 但在东、西和底部的差异不显著。因此, 综合考虑代表性与野外可操作性, 叶片采集首选中胸径成树的底部叶片。随机抽样统计可知, 树木叶面积测定的最适叶片采集数量因物种而异, 木荷的最适叶片采集数量为40, 而枫香树最少为170片。因此, 在叶面积测定时, 叶片采集的数量应该不能只局限在10-20片, 在人力、物力和时间等条件允许的情况下, 应该尽可能多地测定较多叶片的叶面积。     

Steppe-like grassland as a refuge of the wild edible and medicinal plant species in anthropogenic landscape in northeastern Croatia

Habitat fragmentation and modification have been recognized as the most serious threats to biodiversity. In Croatia, the intensive human activities resulted in the landscape transformation, reduction of grassland areas and loss of species number. Increasingly threatened are the wild edible and medicinal plants which contribute remarkably to the biodiversity of different grassland types. In northeastern Croatia, one of the last remnants of the steppe-like grassland remained preserved in a very complex anthropogenic landscape. We examined the diversity of vascular plants occurring on this small habitat patch. Among the relatively high number of plant taxa (in a total of 177) found during the two years of investigations, most could be used both for human nutrition (28 taxa), and for the treatment of a wide range of diseases (60 taxa), while some taxa have nutritional value for livestock (17 taxa). All 23 recorded taxa with edible and medicinal properties are threatened in Croatia, 21 are threatened across Europe, and two are Red Listed taxa. Our results showed that small and isolated steppe-like grassland represents an important refuge for variety of valuable plant species in intensively used landscapes. Accordingly, such small habitats should be, at least locally, effectively protected.     

A novel insight into the cost–benefit model for the evolution of botanical carnivory

Background The cost–benefit model for the evolution of botanical carnivory provides a conceptual framework for interpreting a wide range of comparative and experimental studies on carnivorous plants. This model assumes that the modified leaves called traps represent a significant cost for the plant, and this cost is outweighed by the benefits from increased nutrient uptake from prey, in terms of enhancing the rate of photosynthesis per unit leaf mass or area (A_N) in the microsites inhabited by carnivorous plants.Scope This review summarizes results from the classical interpretation of the cost–benefit model for evolution of botanical carnivory and highlights the costs and benefits of active trapping mechanisms, including water pumping, electrical signalling and accumulation of jasmonates. Novel alternative sequestration strategies (utilization of leaf litter and faeces) in carnivorous plants are also discussed in the context of the cost–benefit model.Conclusions Traps of carnivorous plants have lower A_N than leaves, and the leaves have higher A_N after feeding. Prey digestion, water pumping and electrical signalling represent a significant carbon cost (as an increased rate of respiration, R_D) for carnivorous plants. On the other hand, jasmonate accumulation during the digestive period and reprogramming of gene expression from growth and photosynthesis to prey digestion optimizes enzyme production in comparison with constitutive secretion. This inducibility may have evolved as a cost-saving strategy beneficial for carnivorous plants. The similarities between plant defence mechanisms and botanical carnivory are highlighted.